Cytokine expression patterns in conjunctiva, Harderian gland and trachea after ocular or oral inoculation with a virulent strain of infectious laryngotracheitis virus (ILTV) (2437)
Poultry Medicine |  Laryngotracheitis
Tuesday |  2:00 PM -  2:15 PM
Henry B. Gonzalez Convention Center||221CD

 

Speaker(s)

Gabriela Beltrán DVM
University of Georgia


Presentation Info

CE Credit(s): 0.30
CE Level: 1

Description


Infectious laryngotracheitis virus primarily infects the upper respiratory tract of chickens. The main sites of ILTV lytic replication are the conjunctiva, nasal cavity and the trachea mucosa. We have previously showed that the route of inoculation greatly alters the replication patterns of virulent ILTV strain 63140.  When strain 63140 was administered via the ocular route viral replication was detected in trachea, conjunctiva and nasal cavity.  In contrast when administered via the nasal or oral routes replication was limited to the nasal cavity.  The nasal cavity, conjunctiva and the Harderian gland are structures that although not anatomically connected to the respiratory system are the first to come in contact with the virus and contain associated lymphoid tissues which play essential roles in induction of local immune responses. The specific objective of this study was to determine how the route of inoculation of virulent ILTV strain 63140 influenced cytokine and Toll like receptors (TLR) gene expression in conjunctiva, Harderian gland and trachea tissues after ocular or oral inoculation.  Relative quantification of host gene expression for type 1 interferon (IFN alpha and beta), type II interferon (IFN gamma), interleukines IL1b, IL6, inducible nitric oxide (iNOS), TLR3, and TLR21 was performed by reverse transcriptase real-time PCR.  Preliminary analysis shows that six hours post oral inoculation significant down-regulation of IFN-alpha and IFN-beta gene expression was detected in trachea of infected chickens. While in trachea of chickens inoculated via the ocular route, 12 hours post –inoculation significant down-regulation of IFN-alpha, IFN-gamma, IL-1b, and TLR3 gene expression was detected.  Further analysis of cytokine gene expression in Harderian gland and conjunctiva will be presented.


 

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