A Universal One-Step Real-Time RT-PCR for Detection of all Avian Orthoreovirus Genotypes (2475_2)
Poultry Posters |  Diagnostics
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CE Level: 2

Description

In this paper, we report our recent diagnostic assay development of one-step real-time RT-PCR (rRT-PCR) for the rapid and universal detection of all ARV reference strains and six sC genotypes of the newly emerging field variants in Pennsylvania (PA). This new rRT-PCR detected all six sC genotypes from ARV field variant strains and reference strains tested in this study. There were no cross-reactions with other avian viruses.